|Immunofluorescence (IF)||1:500 - 1:1000|
|Immunoprecipitation (IP)||5 µg|
|Neutralization (Neu)||Assay dependent|
|Western Blot (WB)||1:2000 - 1:4000|
|Tested Species reactivity||Luciferase|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Purified full length recombinant Red Firefly luciferase expressed in E. coli.|
|Storage buffer||PBS with 1mg/ml BSA, 30% glycerol|
|Contains||0.05% sodium azide|
Western blot analysis of PA1-179 detects a prominent ~62 kDa protein in Red Firefly luciferase expressing cells. No signal was detected in Green Renilla transfected or untransfected control HEK293 cells. This antibody has also been successfully tested in IP, Immunofluorescence and neutralization assays.
Red Firefly luciferase activity can be measured using the Thermo Fisher Firefly Luciferase Flash
or Glow Assay Kits or Luciferase Dual Assay Kits.
Firefly luciferase is a 62kDa protein produced in nature by several species of the Lampyridae family of beetles, which includes the genera Photinus and Luciola. Thermo Scientific Red Firefly Luciferase has an emission range of 560-700nm (lambda max = 613nm). Bioluminescent signal from firefly luciferase originates from the oxidation of D-Luciferin. Light output captured using a luminometer can be correlated with the amount of firefly luciferase protein produced and used to determine the activity of the promoter driving firefly luciferase expression. Firefly luciferase bioluminescence provides researchers with a non-radioactive assay for measuring transcriptional activity of regulatory elements. Firefly luciferase reporter activity can be used to determine promoter activity, to study upstream pathway activity in the cell, or to determine effect of activators or inhibitors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Firefly Luciferase; Genji firefly; Japanese firefly; Luciferin 4-monooxygenase; Photinus pyralis; Red-shifted Firefly Luciferase
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