|Flow Cytometry (Flow)||Assay-dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1-2 µg/ml|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
|Immunohistochemistry (IHC)||See 11 publications below|
|Western Blot (WB)||See 5 publications below|
|Immunocytochemistry (ICC)||See 1 publications below|
|Tested Species reactivity||Human, Mouse|
|Published species reactivity||Rat , Human Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant human Rb protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4° C|
MA5-11387 targets Retinoblastoma in FACS, ICC/IF, IHC (P), IP, and WB applications and shows reactivity with human and mouse samples. Does not react with rat samples.
The MA5-11387 immunogen is recombinant human Rb protein.
Rb is a tumor suppressor nuclear phosphoprotein capable of binding to DNA. It is phosphorylated on serine and threonine, but not on tyrosine residues. It forms a complex with SV40 large T antigen, adenovirus E1A, and human papilloma virus-16 E. Rb protein may act by regulating transcription and loss of its function leads to uncontrolled cell growth. Aberrations in the RB gene have been implicated in cancers of breast, colon, prostate, kidney, nasopharynx, and leukemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: exon 17 tumor GOS561 substitution mutation causes premature stop; GOS563 exon 17 substitution mutation causes premature stop; p105-Rb; pp105; pp110; pRb; prepro-retinoblastoma-associated protein; protein phosphatase 1, regulatory subunit 130; Rb; retinoblastoma 1; retinoblastoma suspectibility protein; Retinoblastoma-associated protein
Gene Aliases: OSRC; p105-Rb; pp110; PPP1R130; pRb; RB; Rb-1; RB1
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