|Gel Shift (GS)||1-2 µl|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:500-1:5000|
|Western Blot (WB)||See 1 publications below|
|Immunohistochemistry (IHC)||See 1 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Non-human primate Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues R(13) R L Y G A A G P G A G L L R R(28) of the N-terminal sequence of RAR gamma-2.|
|Storage buffer||whole serum diluted in PBS|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA3-814 detects recombinant human retinoic acid receptor (RAR) gamma 2. The immunizing sequence is 100% conserved in mouse. This antibody does not detect RAR gamma-1, or either of the alpha or beta forms of RAR.
PA3-814 has been successfully used in WB, immunoprecipitation and gel shift procedures. In super shift assays this antibody incompletely shifts human RAR gamma-2/retinoid X receptor (RXR)/RAR response element complexes using various synthetic and natural RARE's.
PA3-814 immunizing peptide corresponds to the N-terminus of RAR gamma-2. This antibody does not detect RAR gamma-1, or either of the alpha or beta forms of RAR. This peptide (Cat. # PEP-229) is available for use in neutralization and control experiments.
Retinoic acid, a metabolite of vitamin A, is necessary for normal organogenesis but acts as a teratogen at high levels during embryonic and fetal development. Retinoic acid functions through its interaction with the nuclear protein, retinoic acid receptor (RAR). RAR belongs to the steroid and thyroid hormone superfamily of nuclear receptor proteins which exert their effects by binding to specific DNA response elements, thus regulating gene expression in target cells. RAR exists as three major subtypes: alpha, beta and gamma. Two main isoforms of RAR gamma exist: RAR gamma-1 has been found to be predominantly expressed in skin, while RAR gamma-2 has been found early in embryogenesis and in embryonal carcinoma cells. RAR gamma is also known as RARD.
The corresponding gene for the retinoic acid receptor gamma 2 is NR1B3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Nuclear receptor subfamily 1 group B member 3; RAR Gamma-2; RAR-gamma; retinoic acid nuclear receptor gamma variant 1; retinoic acid nuclear receptor gamma variant 2; Retinoic acid receptor gamma; retinoic acid receptor, gamma
Gene Aliases: NR1B3; RARC; RARG
UniProt ID: (Human) P13631
Entrez Gene ID: (Human) 5916
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