|Gel Shift (GS)||Assay dependent|
|Immunofluorescence (IF)||Assay dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||5 µg/ml|
|Gel Shift (GS)||See 4 publications below|
|Western Blot (WB)||See 1 publications below|
|Tested Species reactivity||Human, Mouse|
|Published species reactivity||Rabbit , Non-human primate , Human Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant human retinoic X receptor beta protein.|
|Storage buffer||ascites diluted in PBS|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA3-812 detects retinoid X receptor (RXR) beta from human and mouse tissues. This antibody is specific for the RXR beta isoform and does not cross react with either RXR alpha or gamma.
MA3-812 has been successfully used in Western blot, immunofluorescence, immunoprecipitation and gel shift procedures. By Western blot, this antibody detects an ~50 kDa protein representing RXR beta from transfected Sf9 cells. Immunofluorescence staining of RXR beta in MCF-7 cells overexpressing RXR beta with MA3-812 results in strong nuclear staining. Gel super shift experiments show disruption of vitamin D receptor (VDR)/RXR beta/VDRE (DNA) complex formation when RXR beta was preincubated with MA3-812, yet experiments using THR showed a supershift of the thyroid hormone receptor (THR)/RXR beta heterodimer by this product.
The MA3-812 antigen is recombinant human RXR beta.
Retinoid X receptors (RXRs) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptor proteins which exert their effects by binding to specific DNA response elements, thus regulating gene expression in target cells. The RXR subfamily consists of at least three similar genes, RXR alpha, RXR beta and RXR gamma, all of which control transcription of target genes mediated by retinoids. RXR beta controls expression of many genes that respond to hormones and vitamins, including thyroid hormone, estrogen, retinoids and vitamin D. RXR beta, also known as RCoR1, controls a wide array of genes because of its ability to heterodimerize with other hormone receptors including the thyroid hormone receptor (THR), vitamin D receptor (VDR) and retinoic acid receptor (RAR).
The corresponding gene for the retinoid X receptor beta is NR2B2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: MHC class I promoter binding protein; MHC class I regulatory element-binding protein H-2RIIBP; Nuclear receptor subfamily 2 group B member 2; putative retinoid X receptor beta; Retinoic acid receptor RXR-beta; Retinoid X receptor beta; retinoid X receptor, beta; RXR Beta
Gene Aliases: AL023085; DAUDI6; H-2RIIBP; NR2B2; RCoR-1; Rub; RXRB
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