|ELISA (ELISA)||Assay dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1 ug/ml|
|Immunoprecipitation (IP)||5 ug/ml|
|Western Blot (WB)||0.1 ug/ml|
|ELISA (ELISA)||See 1 publications below|
|Western Blot (WB)||See 1 publications below|
|Tested Species reactivity||Mouse, Pig, Rat, Bovine, Human|
|Published species reactivity||Human Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Purified S100-alpha protein from human pectoral muscle cells.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 15mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-932 detects S100-alpha protein in bovine, human, mouse, porcine, and rat samples. This antibody has shown no cross-reactivity with the S100-beta protein.
PA1-932 has successfully been used in Western blot, ELISA, immunofluorescence, immunocytochemistry, immunoprecipitation and immunohistochemical procedures. By Western blot, this antibody detects a 10 kDa protein representing S100-alpha from human muscle. Immunohistochemical staining on paraffin sections demonstrates that S100-alpha is localized in serous cells, myoepithelial cells and epithelial cells of intercalated ducts in the parotid gland after staining with PA1-932.
S100 protein is relatively small and, therefore, it is recommended that the electrophoresis be performed using tricine-SDS-PAGE gels and transferred to a nylon membrane.
The PA1-932 immunizing protein corresponds to purified S100-alpha protein from human pectoral muscle cells.
The S100 protein is a low-molecular-weight, acidic and calcium binding protein that in functional form exist in dimers. S100 has two subunits: S100-alpha (94 aa; human chromosome 1) and S100-beta (92 aa; human chromosome 21) that forms as either homodimers (alpha-alpha known as S-100a(0) or beta-beta known as S-100b) or as heterodimers (known as S-100a) of ~21 kDa. S100-alpha and -beta chains show ~58% sequence identity and are both highly conserved among species. S100-alpha was originally believed to be localized to the CNS, but studies have shown it to be found in numerous tissues including cardiac, skeletal and vascular smooth muscle cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Protein S100-A1; S-100 protein alpha chain; S-100 protein subunit alpha; S100 alpha; S100 calcium-binding protein A1; S100 protein, alpha polypeptide; S100alpha
Gene Aliases: AI266795; S100; S100-alpha; S100A; S100A1
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.