|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:100|
|Western Blot (WB)||2-3 ug/ml|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Peptide corresponding to amino acids 18–40 of human S100B|
|Contains||0.09% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
This antibody is predicted to react with non-human primate and rabbit based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
S100 calcium binding protein B protein family. S100B is present in the cytoplasm and nucleus of various cell types. It plays a role in intracellular Ca2+-dependent functions through regulation of calcium homeostasis, and transduction of intracellular Ca2+ signals. S100B is involved in the regulation of the cell cycle, differentiation, and apoptosis. It also is involved in cell motility through modulation of membrane–cytoskeletal interactions. In humans, the S100B gene is present at the 21q22 locus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: NEF; Protein S100-B; S-100 calcium-binding protein, beta chain; S-100 protein beta chain; S-100 protein beta subunit; S-100 protein subunit beta; S100 calcium-binding protein B; S100 calcium-binding protein beta (neural); S100 calcium-binding protein, beta (neural); S100 protein, beta polypeptide, neural; S100B
Gene Aliases: AI850290; Bpb; NEF; S100; S100-B; S100B; S100beta; S100P
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