|Immunohistochemistry (Paraffin) (IHC (P))||1:200|
|Western Blot (WB)||1:25|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein encoding full length human S100A4.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is tonsil or melanoma tissue.
S100 belongs to the family of calcium binding proteins such as calmodulin and troponin C. S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains. S100 protein is also expressed in the antigen presenting cells such as the Langerhans cells in skin and interdigitating reticulum cells in the paracortex of lymph nodes.
Protein Aliases: 18A2; 42A; Calvasculin; CAPL; fibroblast-specific protein-1; FSP1; leukemia multidrug resistance associated protein; malignant transformation suppression 1; Metastasin; MTS1; murine placental homolog; P9KA; Placental calcium-binding protein; pr; Protein Mts1; Protein S100-A4; S100 calcium-binding protein A4; S100 calcium-binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog); S100 calcium-binding protein A4 calcium protein
Gene Aliases: 18A2; 42A; CAPL; FSP1; MTS1; P9KA; PEL98; S100A4
UniProt ID: (Human) P26447
Entrez Gene ID: (Human) 6275