|Western Blot (WB)||0.5-2 µg/ml|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues 160-210 of human SAE2.|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
Suggested positive control: Hela whole cell extract.
Covalent attachment of one protein to another is one of the more prominent posttranslational modifications in respect to size and ubiquity. Ubiquitin is the most familiar of the protein modifiers and its activation and transfer to target proteins has been studied extensively. Recently, a new group of ubiquitin-like proteins has been receiving a lot of attention. SUMO, or Sentrin, is one of the most intriguing. SUMO has been implicated in the stabilization of target proteins and/or their localization to sub-cellular complexes. The conjugation of SUMO-1, SUMO-2 and SUMO-3 onto target proteins requires the concerted action of the specific E1-activating enzyme SAE1/SAE2, the E2-conjugating enzyme Ubc9, and an E3-like SUMO ligase.
Protein Aliases: Anthracycline-associated resistance ARX; FLJ13058; HRIHFB2115; SUMO-1 activating enzyme subunit 2; SUMO-activating enzyme subunit 2; SUMO1 activating enzyme subunit 2; UBA2, ubiquitin-activating enzyme E1 homolog; ubiquitin-like 1 (sentrin) activating enzyme E1B; ubiquitin-like 1 (sentrin) activating enzyme subunit 2; Ubiquitin-like 1-activating enzyme E1B; ubiquitin-like modifier activating enzyme 2; Ubiquitin-like modifier-activating enzyme 2
Gene Aliases: AA986091; ARX; HRIHFB2115; SAE2; UBA1; UBA2; Ubl1a2; UBLE1B