|Immunocytochemistry (ICC)||Assay dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||0.5 µg/ml|
|Tested Species reactivity||Human|
|Host / Isotype||Mouse / IgG|
|Immunogen||synthetic peptide CPEARDKEDGRKF, corresponding to amino acids 345 to 357 of SAFB.|
|Storage buffer||0.1M tris glycine, pH 7.4, with 0.15M NaCl, 30% glycerol|
|Contains||0.05% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
MA1-91526 detects SAFB from human samples.
MA1-91526 has been successfully used in immunocytochemistry, immunohistochemistry (paraffin tissue), immunoprecipitation, Western blot applications.
The MA1-91526 immunogen is: Synthetic peptide: CPEARDKEDGRKF, corresponding to amino acids 345 to 357 of SAFB.
This gene encodes a DNA-binding protein that has high specificity for scaffold or matrix attachment region DNA elements (S/MAR DNA). This protein is thought to be involved in attaching the base of chromatin loops to the nuclear matrix but there is conflicting evidence as to whether this protein is a component of chromatin or a nuclear matrix protein. Scaffold attachment factors are a specific subset of nuclear matrix proteins (NMP) that specifically bind to S/MAR. This encoded protein is thought to serve as a molecular base to assemble a 'transcriptosome complex' in the vicinity of actively transcribed genes. It is involved in the regulation of the heat shock protein 27 transcription and also can act as an estrogen receptor corepressor. This gene is a candidate gene for breast tumorigenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: DKFZp779C1727; glutathione S-transferase fusion protein; heat-shock protein (HSP27) estrogen response element and TATA box-binding protein; HET; Hsp27 ERE-TATA binding protein; HSP27 ERE-TATA-binding protein; HSP27 estrogen response element-TATA box-binding protein; SAF-B; SAFB1; Scaffold attachment factor B1
Gene Aliases: HAP; HET; SAB-B1; SAF-B; SAF-B1; SAFB; SAFB1
UniProt ID: (Human) Q15424
Entrez Gene ID: (Human) 6294
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