|Western Blot (WB)||Assay-dependent|
|Tested Species reactivity||Amphibian, Rat, Fruit fly, Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Partially purified mammalian spliceosomes.|
|Contains||15mM sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
The antibody labels SC-35 as a speckled pattern in the nucleoplasm, excluding the nucleoli.
Other uses include inhibition and depletion of splicing activity in nuclear extracts and immunoaffinity purification.
No antigen retrieval is necessary.Block in normal serum 10% in TBS (pH 7.6) for 30 min followed by incubation overnight at 4C.
Use ABC amplification- DAB detection.
In Western blot, this antibody detects a doublet of approximately 35 kDa.
Suggested positive control: cultured human fibroblasts.
SC35 is involved in pre-mRNA splicing and is found in the bodies in the nucleus referred to as speckles, SC 35 domains or splicing factor compartments (SFCs). The SC-35 clone is frequently used as a marker for these bodies, the role of which is controversial. Speckles correspond largely if not entirely to ultrastructures termed interchromatin granule clusters (IGCs). SC35 is excluded from the nucleolus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: SC-35; serine/arginine-rich splicing factor 2; splicing component, 35 kDa; splicing factor SC35; splicing factor, arginine/serine-rich 2; SR splicing factor 2
Gene Aliases: PR264; SC-35; SC35; SFRS2; SFRS2A; SRp30b
Molecular Function: Molecular function unclassified
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.