|Immunocytochemistry (ICC)||2.5 ug/mL|
|Immunofluorescence (IF)||2.5 ug/mL|
|Western Blot (WB)||1 - 2 ug/mL|
|Western Blot (WB)||See 1 publications below|
|Tested Species reactivity||Human, Mouse, Rat|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A 15 amino acid synthetic peptide near the center of human SDHD|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
A suggested positive control is EL4 cell lysate.
PA5-34387 can be used with blocking peptide PEP-1430.
The mitochondrial succinate dehydrogenase complex subunit D (SDHD) is one of four proteins that make up the tricarboxylic cycle enzyme succinate dehydrogenase (SCH). Studies have shown that mutations in SDHD often leads to hereditary paragangliomas, usually benign tumors of the autonomic nervous system, suggesting that SDHD also plays a role as a tumor-suppressor gene. In one family with a nonsense mutation (R22X) in the SDHD gene, a loss of heterozygosity was found in the paragangliomas, and within these tumors the enzymatic activity of Complex II in the mitochondrial respiratory chain was completely abolished. Furthermore, high levels of angiogenic factors EPAS1 and VEGF was observed, which may stimulate tumor growth.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CBT1; CII-4; CybS; PGL; PGL1; QPs3; SDH4; SDHD; Succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial; Succinate dehydrogenase complex subunit D; succinate dehydrogenase complex subunit D integral membrane protein; succinate dehydrogenase cytochrome b small subunit mitochondrial; Succinate-ubiquinone oxidoreductase cytochrome b small subunit; Succinate-ubiquinone reductase membrane anchor subunit
Gene Aliases: 3110001M13Rik; AVLL5809; C78570; CBT1; CII-4; CWS3; cybS; PGL; PGL1; PRO19626; QPs3; SDH4; SDHD
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