|Western Blot (WB)||1:20,000|
|Western Blot (WB)||See 2 publications below|
|Tested Species reactivity||Dog, Human, Mouse, Pig, Rat|
|Published species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues C(13) V I L P E I Q K P E R K I Q F K E K(31) of canine SEC61 alpha.|
|Storage buffer||whole serum|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA3-014 detects human, canine and porcine, rat and mouse sec61 alpha from microsomes and shows no cross-reactivity with other sec61 isoforms.
PA3-014 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~38 kDa protein representing sec61 alpha from pig microsomes.
The PA3-014 immunogen is a synthetic peptide corresponding to residues C(13) V I L P E I Q K P E R K I Q F K E K(31) of canine sec61 alpha. This sequence is completely conserved between human, mouse, rat, and canine.
The Sec61 complex is responsible for translocation of proteins across the rough endoplasmic reticulum membrane. Sec61 is composed of three subunits: alpha, beta, and gamma; that associate to form a heterotrimer. Multiple heterotrimers associate forming the ER translocon. Sec61 alpha is a polytopic protein that has ten membrane-spanning loops.
Sec61 has been found to be tightly associated with membrane bound ribosomes, either directly or through adaptor proteins, and is required for assembly of membrane and secretory proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: protein transport protein SEC61 alpha subunit; protein transport protein Sec61 subunit alpha; Protein transport protein Sec61 subunit alpha isoform 1; Sec61 alpha 1 subunit; Sec61 alpha subunit homolog; Sec61 alpha-1; Sec61 Complex Alpha Subunit; sec61 homolog; sec61 homologue; SEC61, alpha subunit; transport protein sec61 alpha subunit
Gene Aliases: AA408394; AA410007; HSEC61; SEC61; SEC61A; SEC61A1
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