|Western Blot (WB)||1:500|
|Western Blot (WB)||See 1 publications below|
|Immunohistochemistry (IHC)||See 1 publications below|
|Tested Species reactivity||Mouse, Rat|
|Published species reactivity||Dog Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic Peptide: V(29) T D A R E R Y G P N(39).|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-910A detects sarcoplasmic or endoplasmic reticulum calcium ATPase 3 (SERCA3) mouse and rat samples. This antibody does not detect other SERCA isoforms.
PA1-910A has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~97 kDa protein representing recombinant SERCA3 from rat samples.
PA1-910A immunizing peptide corresponds to amino acid residues 29-39 from rat and mouse SERCA3. PA1-910A immunizing peptide (Cat. # PEP-015) is available for neutralization and control experiments.
ATP dependent calcium pumps are responsible in part for the maintenance of low cytoplasmic free calcium concentrations. The ATP pumps that reside in intracellular organelles are encoded by a family of structurally related enzymes, termed the sarcoplasmic or endoplasmic reticulum calcium ATPases (SERCA). The SERCA2 gene is subject to tissue dependent processing which is responsible for the generation of SERCA2a muscle-specific isoform expressed in type I (slow) skeletal, cardiac and smooth muscle and the SERCA2b isoform expressed in all cell types. SERCA3 has been found to co-express with SERCA2b in platelets, mast cells, lymphoid cells, and epithelial cells. Due to this pattern of expression it is referred to as the non-muscle form of SERCA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Calcium pump 3; sarcoendoplasmic reticulum Ca2+ ATPase type 3; Sarcoplasmic or Endoplasmic Reticulum Calcium ATPase 3; Sarcoplasmic/endoplasmic reticulum calcium ATPase 3; SERCA3; SR Ca(2+)-ATPase 3
Gene Aliases: Atp2a3; SERCA3; SERCA3b
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