|ChIP assay (ChIP)||1:10-1:500|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Western Blot (WB)||1:500-1:1000|
|Tested Species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthethic peptides corresponding to residues G(131) E V N E D G E M T G E K I A(145) and G(336) Y D H S P P G K S G P E A P E W(352) of human Set7/9.|
|Storage buffer||whole serum diluted in PBS|
|Contains||0.05% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
These sequences are 100% conserved between human, mouse and rat.
Suggested positive control: Hela whole cell extract, HeLa cell lysate.
Diverse signal transduction pathways impinging on the N-terminal tails of histones lead to a number of post-translational modifications including acetylation, phosphorylation, poly(ADP-ribosylation), ubiquitination and methylation. These modifications play critical roles in regulating chromatin structure and gene expression. Histone methyltransferases (HMTases) selectively methylate evolutionarily conserved arginine or lysine residues, primarily in the N-terminal tails of histones H3 and H4. Set7/9 is a histone specific HMTase that methylates histone H3 lysine 4. Set7/9 transfers methyl groups to lysine 4 of histone H3 in complex with S-adenosyl-L-methionine. Human Set7/9 is a 336 amino acid protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: H3-K4 methyltransferase; H3-K4-HMTase SETD7; Histone H3-K4 methyltransferase SETD7; histone H3-lysine 4-specific methyltransferase; Histone-lysine N-methyltransferase SETD7; Lysine N-methyltransferase 7; SET domain containing (lysine methyltransferase) 7; SET domain-containing protein 7; SET7/9
Gene Aliases: 1600028F23Rik; H3K4MT; KIAA1717; KMT7; mKIAA1717; SET7; SET7/9; SET9; SETD7
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