|Immunohistochemistry (Paraffin) (IHC (P))||1:30|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to aa 576-593 of human SH-PTP2 protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is bone marrow tissue.
The steady state of protein tyrosyl phosphorylation in cells is regulated by the opposing action of tyrosine kinases and protein tyrosine phosphatases (PTPs). Several groups have independently identified a non-transmembrane PTP, designated SH-PTP1 (also known as PTP1C, HCP and SHP), which is primarily expressed in hematopoietic cells and characterized by the presence of two SH2 domains N-terminal to the PTP domain. A second and much more widely expressed PTP with SH2 domains, SH-PTP2 (also designated PTP1D and Syp), has been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: BPTP3; NS1; Protein-tyrosine phosphatase 1D; Protein-tyrosine phosphatase 2C; PTP-1D; PTP-2C; PTP2C; SH-PTP2; SH-PTP3; SHP-2; SHP2; Tyrosine-protein phosphatase non-receptor type 11
Gene Aliases: BPTP3; CFC; JMML; METCDS; NS1; PTP-1D; PTP2C; PTPN11; SH-PTP2; SH-PTP3; SHP2; SHPTP2
UniProt ID: (Human) Q06124
Entrez Gene ID: (Human) 5781
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