|Immunohistochemistry (IHC)||5 ug/ml|
|Western Blot (WB)||1-2 ug/ml|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A 21 amino acid peptide near the amino terminus of human SHOC2.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
A suggested positive control is Jurkat cell lysate.
PA5-20762 can be used with blocking peptide PEP-0876.
SHOC2 protein participates in protein binding / transferase activity in the fibroblast growth factor receptor signaling pathway and Ras protein signal transduction. It is a widely expressed protein composed almost entirely of leucine-rich repeats (LRR), with a lysine-rich sequence at the amino terminus and cytoplasmically localized. SHOC2 acts as a positive modulator of the RAS-MAPK signaling cascade, which is elicited by EGL-15 and LET-23 and mediated by LET-60. SHOC2 together with protein phosphatase 1c (PP1c) forms a highly specific M-Ras effector complex and is essential for activation of the MAPK pathway by growth factors. Furthermore, in tumor cells with Ras gene mutations, inhibition of SHOC2 expression inhibits MAPK, but not PI3K activity. The SHOC2-PP1c holoenzyme provides an attractive therapeutic target for inhibition of the MAPK pathway in cancer. Recent studies show that aberrantly acquired N-myristoylation of SHOC2 causes human disease Noonan-like syndrome with loose anagen hair.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Leucine-rich repeat protein SHOC-2; Protein soc-2 homolog; Protein sur-8 homolog; Ras-binding protein SUR-8; RP11-348N5.2, SIAA0862, SOC2, SUR8; SHOC2 leucine-rich repeat scaffold protein; soc-2 (suppressor of clear) homolog; soc-2 suppressor of clear homolog; suppressor of clear, C. elegans, homolog of
Gene Aliases: AU017287; KIAA0862; mKIAA0862; SHOC2; SIAA0862; SOC2; SUR8
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