|Western Blot (WB)||Assay dependent|
|Tested Species reactivity||Bovine, Dog, Human, Mouse, Pig, Rat|
|Host / Isotype||Goat / IgG|
|Immunogen||synthetic peptide SRCGELLPLIDPC.|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-31010 detects SIRT4 from human samples. Expected to cross-react with mouse and rat samples due to sequence homology.
PA1-31010 has been successfully used in Western blot applications. PA1-31010 detects a band of ~40 kDa in human kidney and U937 lysates.
The PA1-31010 immunogen is: Synthetic peptide: SRCGELLPLIDPC.
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family.
Protein Aliases: MGC130046; MGC130047; MGC57437; NAD-dependent ADP-ribosyltransferase sirtuin-4; NAD-dependent deacetylase sirtuin-4; NAD-dependent protein deacetylase sirtuin-4; NAD-dependent protein lipoamidase sirtuin-4, mitochondrial; Regulatory protein SIR2 homolog 4; sir2-like 4; SIR2-like protein 4; SIR2L4; SIRT4; sirtuin type 4
Gene Aliases: 4930596O17Rik; BOS_16541; SIR2L4; SIRT4