|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:200|
|Western Blot (WB)||1:500-1:1000|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to the N-terminal of human Smad2|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.2|
|Contains||15mM sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
This antibody detects endogenous protein at a molecular weight of 55 and 60 kDa.
Purity is >95% by SDS-PAGE.
Smad proteins, the mammalian homologs of the Drosophila mothers against decapentaplegic (Mad), have been implicated as downstream effectors of GF beta/BMP signaling. Smad1 (also designated Madr1 or JV4-1) and Smad5 are effectors of BMP-2 and BMP-4 function, while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGF beta and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGF beta signaling by interfering with TGF beta-mediated phosphorylation of other Smad proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: hMAD-2; hMAD-3; hSMAD2; hSMAD3; JV15-2; JV18-1; MAD (mothers against decapentaplegic, Drosophila) homolog 3; MAD homolog 2; MAD homolog 3; mad homolog JV15-2; mad protein homolog; MAD, mothers against decapentaplegic homolog 3; Mad-related protein 2; Mad3; MADH2; MADH3; MADR2; mMad2; mMad3; mother against DPP homolog 2; Mothers against decapentaplegic homolog 2; Mothers against decapentaplegic homolog 3; Mothers against DPP homolog 2; Mothers against DPP homolog 3; Sma- and Mad-related protein 2; SMA- and MAD-related protein 3; SMAD 2; SMAD 3; SMAD family member 2; SMAD family member 3; SMAD, mothers against DPP homolog 2; SMAD, mothers against DPP homolog 3; SMAD2; SMAD3
Gene Aliases: 7120426M23Rik; AU022421; hMAD-2; hSMAD2; HSPC193; HsT17436; JV15-2; JV18; JV18-1; LDS1C; LDS3; mad3; MADH2; MADH3; MADR2; mMad2; Smad 3; Smad-2; SMAD2; SMAD3
Molecular Function: transcription factor
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