|Immunocytochemistry (ICC)||1-2 ug/ml|
|Immunofluorescence (IF)||1-2 µg/ml|
|Western Blot (WB)||0.5-1 µg/ml|
|Tested Species reactivity||Fish, Human, Mouse, Zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide, conjugated to KLH, corresponding to residues A(294) L K L C S E L G L G G E F(307) of human SMARCB1 with N-terminally added Cystein|
|Purification||Antigen affinity chromatography|
|Contains||15mM sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-25450 detects SMARCB1 from human samples. PA1-25450 is expected to cross react with mouse (100% conserved) and zebrafish (100% conserved) due to sequence homology.
PA1-25450 has been successfully used in Western blot procedures.
The PA1-25450 immunogen is a synthetic peptide, conjugated to KLH, corresponding to residues A(294) L K L C S E L G L G G E F(307) of human SMARCB1 with N-terminally added Cysteine.
The protein encoded by this gene is part of a complex that relieves repressive chromatin structures, allowing the transcriptional machinery to access its targets more effectively. The encoded nuclear protein may also bind to and enhance the DNA joining activity of HIV-1 integrase. This gene has been found to be a tumor suppressor, and mutations in it have been associated with malignant rhabdoid tumors. Two transcript variants encoding different isoforms have been found for this gene.
Protein Aliases: BAF47; BRG1-associated factor 47; hSNF5; hSNFS; INI1; Integrase interactor 1 protein; malignant rhabdoid tumor suppressor; mSNF5; protein phosphatase 1, regulatory subunit 144; RDT; Sfh1p; SNF5; SNF5 homolog; SNF5L1; Snr1; sucrose nonfermenting, yeast, homolog-like 1; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1-A; SWI/SNF-related matrix-associated protein
Gene Aliases: AU020204; BAF47; CH211-251J10.2; CSS3; hSNFS; INI1; MRD15; PPP1R144; RDT; RTPS1; Sfh1p; SMARCB1; smarcb1a; SNF5; SNF5/INI1; SNF5L1; Snr1; SWNTS1; zgc:92517