|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1-2 µg/ml|
|Western Blot (WB)||See 3 publications below|
|Tested Species reactivity||Human, Mouse, Rat|
|Published species reactivity||Bovine , Mouse Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Sythetic Peptide: C N(193) K N R I D I A N T R A K K L I D S(210)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-738 detects synaptosomal-associated protein 23 (SNAP-23) from human, mouse and rat samples.
PA1-738 has been successfully used in Western blot and immunoprecipitation procedures. By Western blot, this antibody detects an ~23 kDa protein representing SNAP-23 from rat/mouse brain protein extract and HeLa cell lysates.
PA1-738 immunizing peptide corresponds to amino acid residues 193-210 from mouse SNAP-23 protein. PA1-738 immunizing peptide (Cat. # PEP-147) is available for use in neutralization and control experiments.
Synaptosomal-associated proteins (SNAPs) are cytosolic proteins that play a key role in in the process of membrane fusion in intracellular vesicle trafficking. In eukaryotic cells, the SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) complex is critical to membrane docking and fusion and is believed to impart some degree of specificity between vesicle SNARE (v-SNARE) and target organelle SNARE (t-SNARE). In neurons and neuroendocrine cells, the SNARE complex consists of the integral membrane proteins VAMP (vesicle-associated membrane protein), syntaxin and SNAP-25. In non-neuronal tissue, a SNAP-25 homolog, SNAP-23, functionally replaces SNAP-25 in the SNARE complex. Studies show that VAMP, syntaxin and SNAP-23 are required for SNARE function and that this complex exists as a heterotrimer of the three proteins. In insulin signalling pathways, studies suggest that the translocation of GLUT4 in adipocytes requires that functional SNAP-23 be present in SNARE complexes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: SNAP-23; Synaptosomal-Associated Protein 23; synaptosomal-associated protein 23 kD; synaptosomal-associated protein, 23 kD; synaptosomal-associated protein, 23kD; synaptosomal-associated protein, 23kDa; synaptosome associated protein 23kDa; Syndet; Vesicle-membrane fusion protein SNAP-23
Gene Aliases: 23kDa; AA408749; HsT17016; SNAP-23; SNAP23; SNAP23A; SNAP23B; Sndt; Syndet
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