|Flow Cytometry (Flow)||1:100|
|Immunohistochemistry (Frozen) (IHC (F))||1:50-1:500|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:500|
|Immunoprecipitation (IP)||5µg, 1:1000|
|Western Blot (WB)||1:1000|
|Immunocytochemistry (ICC)||See 1 publications below|
|Tested Species reactivity||Fish, Human, Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Full-length human recombinant protein expressed in bacteria|
|Storage buffer||PBS with 1mg/ml BSA, 30% glycerol|
|Contains||0.05% sodium azide|
PA1-094 detects SOX2 in human and mouse samples and has been successfully used in Western blot, immunofluorescence, IHC (P), FACS and immunoprecipitation applications. Western blot analysis of PA1-094 specifically detects SOX2 protein at ~36 kDa in the nucleus of human and mouse embryonal carcinoma and iPS cells. Upon overexposure of western blots, additional higher and lower molecular weight bands may appear. This antibody has also been successfully used in IHC to detect SOX2 in frozen sections of Knifefish brain samples.
This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ANOP3; MCOPS3; sex determining region Y box 2; sex determining region Y-box 2; SRY (sex determining region Y)-box 2; SRY box 2; SRY-box containing gene 2; SRY-related HMG-box gene 2; Transcription factor SOX-2; transcription factor SOX2
Gene Aliases: ANOP3; lcc; MCOPS3; Sox-2; SOX2; ysb
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