|Flow Cytometry (Flow)||1:200 - 1:400|
|Western Blot (WB)||1:500 - 1:2000|
|Tested Species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified recombinant fragment of human SPI1 (amino acids: 124-271) expressed in E. Coli.|
|Contains||0.03% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
MA5-17178 targets SPI1 in FACS, pep-ELISA, and WB applications and shows reactivity with Human samples.
The MA5-17178 immunogen is purified recombinant fragment of human SPI1 (amino acids: 124-271) expressed in E. Coli.
MA5-17178 detects SPI1 which has a predicted molecular weight of approximately 42kDa.
This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 31 kDa transforming protein; 31 kDa-transforming protein; hematopoietic transcription factor PU.1; spleen focus forming virus (SFFV) proviral integration oncogene spi1; Transcription factor PU.1
Gene Aliases: OF; PU.1; SFPI1; SPI-1; SPI-A; SPI1
UniProt ID: (Human) P17947
Entrez Gene ID: (Human) 6688
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.