|ChIP assay (ChIP)||1-3 ul|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||5-10 µg/ml|
|Tested Species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein corresponding to residues 321-640 of human SPT16, conjugated to KLH.|
|Purification||Ammonium sulfate precipitation|
|Contains||0.08% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
SPT16 and SSRP1 are found in all eurkaryotic cells and exists as part of an essential heterodimer complex. Together, SPT16 and SSRP1 are a highly abundant nuclear complex that in mammals has been called FACT, or Facilitates Chromatin Transcription. FACT enhances the processivity of RNA polymerase II through nucleosomes. Evidence also points to a role for FACT in replication, possibly by enhancing DNA polymerase through nucleosomes as well.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CDC68; FACT; FACTP140; FLJ10857; FLJ14010; Spermatogenesis-associated protein 16; spermatogenesis-related protein; SUPT16H; testicular tissue protein Li 185; Testis development protein NYD-SP12; testis-specific Golgi protein
Gene Aliases: 4921511F01Rik; 4930503K02Rik; NYD-SP12; SPATA16; SPGF6
Molecular Function: chaperone
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