|Flow Cytometry (Flow)||1:50-1:500|
|Tested Species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgM|
|Immunogen||BALB/c mouse immunized with F9 teratocarcinoma stem cells (X-irradiated).|
|Purification||Affinity chromatography - MBP|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||4° C, do not freeze|
MA1-022-PE has been successfully used in ICC/IF and flow cytometry applications on mouse and human samples.
CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin and acutes disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CD15; ELFT; FCT3A; FUC-TIV; FUTIV; LeX; SSEA-1; SSEA1; stage-specific embryonic antigen 1
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