|ELISA (ELISA)||1 µg/ml|
|Flow Cytometry (Flow)||1:50-1:500|
|Tested Species reactivity||Human|
|Host / Isotype||Rat / IgM|
|Immunogen||Fischer rat immunized with 4-8 cell stage mouse embryos|
|Purification||Affinity chromatography - MBP|
|Storage buffer||PBS with proprietary stabilizer|
|Contains||0.02% sodium azide|
|Storage conditions||4° C, do not freeze|
MA1-020-D488 has been successfully used in ICC/IF and flow cytometry applications on human samples.
MA1-020-D488 can be used for immunofluoresence analysis of SSEA3 in human iPSCs.
SSEA-3 (stage-specific embryonic antigen 3) is a cell surface antigen, expressed along with SSEA-4, TRA-1-60 and TRA-1-81 in embryonic stem cells, embryonal carcinoma cells, and induced pluripotent stem cells (iPS). In human cells, these surface markers are down-regulated during the differentiation process, while SSEA1 is absent in undifferentiated human stem cells and upregulated on the cell surface after retinoic acid mediated differentiation. Conversely, in mouse stem cells, SSEA-1 expression decreases with differentiation while SSEA-3 and SSEA-4 expression increase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: SSEA-3; stage-specific embryonic antigen 3
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