|Flow Cytometry (Flow)||1:50-1:100|
|Miscellaneous PubMed (MISC)||See 2 publications below|
|Immunocytochemistry (ICC)||See 2 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG3|
|Immunogen||human embryonal carcinoma cell line 2102Ep|
|Storage buffer||potassium phosphate with 0.15M NaCl, 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA1-021X has been successfully used in immunofluorescence and flow cytometry applications on human samples.
Stage-specific embryonic antigen 4 (SSEA-4) is a cell surface antigen, expressed along with SSEA-3, TRA-1-60 and TRA-1-81 in embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). In human cells, these surface markers are down-regulated during the differentiation process, while SSEA-1 is absent in undifferentiated human stem cells and upregulated on the cell surface after retinoic acid mediated differentiation. Conversely, in mouse stem cells, SSEA-1 expression decreases with differentiation while SSEA-3 and SSEA-4 expression increase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: SSEA-4; stage-specific embryonic antigen 4
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