|Immunoprecipitation (IP)||2 µg|
|Western Blot (WB)||1:200-1:1000|
|Tested Species reactivity||Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||A synthetic peptide of STEP46 (amino acids 57-74: MCTPGCNEEGFGYLVSPR)|
|Storage buffer||PBS, pH 7.2, with 30% glycerol, 1mg/ml BSA|
|Contains||0.05% sodium azide|
By Western blot, MA1-158 detects both STEP 46 and STEP 61. By IP, STEP61 was preferentially pulled down, along with a non-specific protein at ~50kDa.
Rat brain lysate can be used as positive control.
Tyrosine phosphorylation plays a critical role in many physiological processes. There is a fine balance between phosphorylation caused by protein tyrosine kinases and actions of protein tyrosine phosphatases, that regulates these physiological processes. Striatal enriched phosphatase (STEP) is part of a family of protein tyrosine phosphatases enriched within the CNS and is a component of the NMDAR complex. It is expressed within dopaminoceptive neurons in the CNS and particularly enriched within the basal ganglia and related structures. STEP61 is a membrane-associated isoform and STEP46 is a cytosolic protein. In response to transient hypoxia-ischemia in the brain, STEP61 is proteolytically cleaved by calpain, thereby releasing a smaller isoform, STEP33.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Neural-specific protein-tyrosine phosphatase; Neural-specific protein-tyrosine phosphatase PTPN5; protein-tyrosine phosphatase striatum-enriched; protein-tyrosine-phosphatase non-receptor 5; PTN5; STEP; Striatum-enriched protein-tyrosine phosphatase; Tyrosine-protein phosphatase non-receptor type 5
Gene Aliases: Ptpn5; STEP
UniProt ID: (Rat) P35234
Entrez Gene ID: (Rat) 29644
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