|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:200|
|Western Blot (WB)||1:500-1:1000|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to the N-terminal of human SYT|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.2|
|Contains||0.05% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
This antibody detects endogenous protein at a molecular weight of 45 kDa.
Purity is >95% by SDS-PAGE.
The transcriptional coactivator SYT (synovial translocation protein) contains a conserved amino terminal SNH domain and a carboxy terminal QPGY domain, which is a functioning transcriptional activating sequence. Synovial sarcoma translocation (SSX) proteins, including SSX1-5, are transcriptional repressors that contain a repressor domain in their carboxy termini. SSX proteins are localized to the nucleus and expressed in testis and several types of cancers and, therefore, they are classified as C/T (cancer/testis) antigens. The t(x;18) translocation results in the fusion of the amino terminus of SYT to the carboxy terminus of either SSX1 or SSX2; both fusions result in the production of transcriptional activators.
Protein Aliases: Protein SSXT; Protein SYT; SS18; SSXT; synovial sarcoma associated SS18-beta; synovial sarcoma associated SS18-delta; synovial sarcoma associated SS18-gamma; Synovial sarcoma translocated to X chromosome protein; synovial sarcoma translocation, chromosome 18; synovial sarcoma, translocated to X chromosome; Synovial sarcoma-associated Ss18-alpha; SYT
Gene Aliases: D130059H17; SS18; SSXT; SYT