|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 202 of Human Securin|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA5-29399 targets Securin in IF, IHC-P, IP and WB applications and shows reactivity with Human samples.
The PA5-29399 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 202 of Human Securin.
The encoded protein is a homolog of yeast securin proteins, which prevent separins from promoting sister chromatid separation. It is an anaphase-promoting complex (APC) substrate that associates with a separin until activation of the APC. The gene product has transforming activity in vitro and tumorigenic activity in vivo, and the gene is highly expressed in various tumors. The gene product contains 2 PXXP motifs, which are required for its transforming and tumorigenic activities, as well as for its stimulation of basic fibroblast growth factor expression. It also contains a destruction box (D box) that is required for its degradation by the APC. The acidic C-terminal region of the encoded protein can act as a transactivation domain. The gene product is mainly a cytosolic protein, although it partially localizes in the nucleus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Esp1-associated protein; ESP1-associated protein 1; HPTTG; Pituitary tumor-transforming gene 1 protein; PTTG; Securin; Tumor-transforming protein 1; TUTR1
Gene Aliases: EAP1; HPTTG; PTTG; PTTG1; TUTR1
UniProt ID: (Human) O95997
Entrez Gene ID: (Human) 9232
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