|Immunohistochemistry (Frozen) (IHC (F))||1:10-1:100|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:100|
|Immunomicroscopy (IM)||Assay dependent|
|Western Blot (WB)||1:100-1:500|
|Immunohistochemistry (Paraffin) (IHC (P))||See 5 publications below|
|Miscellaneous PubMed (MISC)||See 2 publications below|
|Immunohistochemistry (IHC)||See 178 publications below|
|Immunohistochemistry (Frozen) (IHC (F))||See 1 publications below|
|Immunocytochemistry (ICC)||See 27 publications below|
|Western Blot (WB)||See 16 publications below|
|Flow Cytometry (Flow)||See 3 publications below|
|Tested Species reactivity||Many|
|Published species reactivity||Dog , Rabbit , Rat , Pig , Non-human primate , Bovine , Sheep , Cat , Human , Mouse , Rhesus monkey|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Peptide corresponding to residues E(1) E E D S T A L V C(10) of alpha-smooth muscle actin with an acetylated n-terminus coupled to KLH.|
|Contains||0.09% sodium azide|
|Storage conditions||4°C or -20°C if preferred|
MA1-06110 detects a-smooth muscle actin (a-SM1) in human, rat, goat, porcine, non-human primate, quail, ovine, xenopus, monkey and chicken samples. Since the epitope recognized by MA1-06110 is highly conserved, it can also cross-react with protochordates, lower craniates and mammals other than those specifically listed.
MA1-06110 has sucessfully been used in immunocytochemistry, immunomicroscopy, immunohistochemistry, and Western blotting procedures.
The MA1-06110 immunogen is a peptide corresponding to residues E(1) E E D S T A L V C(10) of alpha-smooth muscle actin with an acetylated n-terminus coupled to KLH. MA1-06110 specifically recognizes the epitope Ac-EEED on alpha-smooth muscle actin.
Among the six actin isoforms described in mammals, two are found in virtually all cells (b- and g-cytoplasmic), two are detected in smooth muscle cells (a- and g-smooth muscle) and two are present in striated muscles, one predominantly in skeletal (a-skeletal) and one in cardiac (a-cardiac) muscle cells. These actin isoforms differ slightly in their N-terminus and the sequences of each of them are perfectly conserved in higher vertebrates. Alpha-smooth muscle actin is abundant in vascular and visceral smooth muscle cells. In addition, it has also been shown to appear in stress fibers of fibroblastic cells during pathological situations involving contractile phenomena such as wound healing and fibrocontractive diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: actin, alpha 2, smooth muscle, aorta; actin, alpha, vascular smooth muscle; Actin, aortic smooth muscle; Alpha Actin 2; alpha smooth muscle actin; Alpha-actin; Alpha-actin-2; alpha-cardiac actin; alpha-SM-actin; alpha-sma; alpha-smooth muscle actin; aortic smooth muscle; Cell growth-inhibiting gene 46 protein; SM actin; smooth muscle alpha-actin; vascular smooth muscle alpha-actin
Gene Aliases: 0610041G09Rik; a-SMA; AAT6; ACT-4; ACTA2; ACTSA; ACTVS; alphaSMA; ASMA; BOS_23286; GIG46; MYMY5; sm-actin; SMactin; SMalphaA
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