|Flow Cytometry (Flow)||1:20|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1/100|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:1,000|
|Immunocytochemistry (ICC)||See 7 publications below|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Western Blot (WB)||See 43 publications below|
|Immunohistochemistry (IHC)||See 3 publications below|
|Immunoprecipitation (IP)||See 2 publications below|
|Tested Species reactivity||Dog, Guinea pig, Human, Mouse, Rabbit, Rat|
|Published species reactivity||Dog , Rabbit , Rat , Pig , Rodent , Marsupial , Bovine , Mouse , Human , Guinea pig|
|Host / Isotype||Mouse / IgM|
|Immunogen||Purified canine cardiac sodium/calcium exchanger.|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA3-926 detects sodium/calcium exchanger from human, canine, rabbit, mouse, guinea pig and rat kidney and cardiac tissues.
MA3-926 has been successfully used in Western blot, immunofluorescence, immunohistochemistry, and immunoprecipitation procedures. By Western blot, this antibody detects a 120 kDa protein representing the sodium/calcium exchanger from guinea pig cardiac extract. The bands seen at 70 kDa and 160 kDa represent a proteolytic fragment and non-reduced exchanger respectively. MA3-926 is not recommended for Western blot procedures of rat tissues. Immunohistochemical staining of the sodium/calcium exchanger in rat heart with MA3-926 results in staining of the plasma membrane and intense staining of cardiac T-tubular membrane.
The MA3-926 antigen is purified canine cardiac sodium/calcium exchanger. This antibody recognizes an epitope between amino acids 371-525, which is on the intracellular side of the plasma membrane.
The sodium/calcium exchanger of cardiac sarcolemma rapidly transports calcium during excitation-contraction coupling and is the dominant myocardial calcium efflux mechanism. The sodium/calcium exchanger uses the transmembrane sodium gradient to catalyze countertransport of calcium against its electrochemical gradient in a 3 sodium : 1 calcium exchange. Sodium/calcium exchange activity is present in excitable cells and in non-excitable cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: cardiac sarcolemmal sodium-calcium exchanger; Na(+)/Ca(2+)-exchange protein 1; Na+/C2+ exchanger; Na+/Ca++ exchanger; Na+/Ca2+ exchanger; Na-Ca exchanger; Na/Ca exchanger; NKX; sodium-calcium exchanger; sodium/calcium exchanger; Sodium/calcium exchanger 1; sodium/calcium exchanger isoform NaCa13; sodium/calcium exchanger isoform NaCa3; sodium/calcium exchanger isoform NaCa7; sodium/calcium exchanger isoform NaCa9; solute carrier family 8 (sodium/calcium exchanger), member 1; Solute carrier family 8 member 1; solute carrier family 8, member 1
Gene Aliases: AI852629; AV344025; CNC; D930008O12Rik; Ncx; NCX1; SLC8A1
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