|Immunocytochemistry (ICC)||Assay dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:200|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:1000-1:10,000|
|Western Blot (WB)||See 28 publications below|
|Miscellaneous PubMed (MISC)||See 2 publications below|
|Immunocytochemistry (ICC)||See 2 publications below|
|Tested Species reactivity||Dog, Human, Mouse, Sheep, Pig|
|Published species reactivity||Dog , Fruit fly , Sheep , Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Purified lamb kidney beta sodium/potassium ATPase.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA3-930 detects sodium/potassium ATPase beta 1 from mouse, human, sheep, canine, and pig tissues. This antibody does not recognize the beta 1 or beta 2 subunit in rat.
MA3-930 has been successfully used in Western blot and immunohistochemistry and immunocytochemistry procedures. By Western blot, this antibody detects an ~42 kDa protein in liver samples and ~55 kDa in kidney samples representing beta sodium/potassium ATPase in sheep liver extract. Immunohistochemical staining of the sodium/potassium ATPase with MA3-930 yields a pattern consistent with plasma membrane localization.
The MA3-930 antigen is purified lamb kidney sodium/potassium ATPase beta. This antibody recognizes and epitope between amino acid residues 195-199 of sheep sodium/potassium ATPase beta 1.
The sodium/potassium ATPase is an integral membrane enzyme found in all cells of higher organisms and is responsible for the ATP-dependent transport of sodium and potassium across the cell membrane. This membrane-bound enzyme is related to a number of other ATPases including sarcoplasmic and endoplasmic reticulum calcium ATPase (SERCA) and plasma membrane calcium ATPase (PMCA). The sodium/potassium ATPase consists of a large, multipass, transmembrane catalytic subunit, termed the alpha subunit, and an associated smaller glycoprotein, termed the beta subunit. Studies indicate that there are three isoforms of the alpha subunit (alpha 1, alpha 2, alpha 3) and two isoforms of the beta subunit (beta 1 and beta 2) encoded by two multigene families.
The different isoforms of the sodium/potassium ATPase exhibit a tissue-specific and developmental pattern of expression. The alpha 1 and beta mRNAs are present in all cell types examined whereas the alpha 2 and alpha 3 mRNAs exhibit a more restricted pattern of cell-specific expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: adenosinetriphosphatase; ATPase beta subunit; ATPase, Na+/K+ transporting, beta 1 polypeptide; Beta 1-subunit of Na(+),K(+)-ATPase; Na+, K+-ATPase beta-subunit; Na+/K+ ATPase Beta; Na, K-ATPase beta-1 polypeptide; sodium pump subunit beta-1; sodium-potassium ATPase subunit beta 1 (non-catalytic); sodium/potassium ATPase beta subunit; sodium/potassium-dependent ATPase beta-1 subunit; Sodium/potassium-dependent ATPase subunit beta-1; sodium/potassium-transporting ATPase beta-1 chain; Sodium/potassium-transporting ATPase subunit beta-1
Gene Aliases: ATP1B; ATP1B1; Atp4b; Atpb; Atpb-1; NKbeta1
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