|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Western Blot (WB)||2-3 ug/ml|
|Tested Species reactivity||Human, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Peptide corresponding to amino acids 173–184 of human SHH.|
|Contains||0.09% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale and purified with Protein A.
ABfinity™ oligoclonal antibodies comprise a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds—the sensitivity of a polyclonal antibody with the specificity of a monoclonal, all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody—recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets when compared with monoclonal antibodies—an oligoclonal antibody has a known mixture of light and heavy chains. This exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Sonic Hedgehog (SHH) is a secreted signaling protein belonging to Hedgehog family. It plays an important role in patterning and organogenesis during embryonic development. In adult cells, SHH plays a role in control of cell division. The hedgehog signalling pathway is initiated when SHH binds the Patched receptor (PTC) resulting in release of SMO from repression. SMO acts to inhibit proteolytic cleavage of the Ci protein which acts as a transcriptional activator.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: HHG-1; HHG1; HPE3; MCOPCB5; SHH; SMMCI; sonic hedgehog homolog; Sonic hedgehog protein; TPT; TPTPS
Gene Aliases: HHG1; HLP3; HPE3; MCOPCB5; SHH; SMMCI; TPT; TPTPS; Vhh-1
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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