|Western Blot (WB)||1:400|
|Tested Species reactivity||Many|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Purified, full length, native chicken spectrin alpha and beta chain protein|
|Storage buffer||whole serum|
|Contains||0.1% sodium azide|
|Storage conditions||-20°C or -80°C if preferred|
PA1-25008 detects Spectrin from a wide range of avian and mammalian samples.
PA1-25008 has been successfully used in Western blot and immunofluorescence procedures. The predicted molecular weight of PA1-25008 is 220 and 240 kDa.
The PA1-25008 immunogen is purified, full length, native chicken spectrin alpha and beta chain protein.
Spectrin is the major cytoskeletal component in erythrocytes. It is composed of two types of polypeptides, denoted alpha and beta, with molecular weights of 240,000 and 220,000 daltons, respectively. The alpha and beta chains form heterotetramers which are linked end to end via oligomeric actin and band 4.1 protein, forming an elaborate submembranal network. The spectrin web is further attached to the erythrocyte membrane via another protein, namely ankyrin. In recent years a diverse number of spectrin like molecules have been found in non-erythroid cells. In most cases the different spectrin-related proteins contain the common alpha chain and a tissue specific beta chain. The spectrin-like molecules in non-erythroid cells are known by different names such as fodrin, CBP I, calspectin and TW 260/240. It should be noted that spectrins bind calmodulin.
Protein Aliases: alpha-I spectrin; alpha-spectrin 1, erythroid; erythroid alpha-spectrin; hemolytic anemia; neuroscience mutagenesis facility, 4; spectrin alpha 1; spectrin alpha chain, erythrocyte; spectrin alpha chain, erythrocytic 1; spherocytosis
Gene Aliases: AF093576; AI451697; EL2; ha; HPP; HS3; ihj; nmf4; sph; SPH3; Spna-1; Spna1; SPTA