|Western Blot (WB)||2 µg/ml|
|Western Blot (WB)||See 1 publications below|
|Tested Species reactivity||Human, Mouse, Sheep, Rat|
|Published species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues 200-286 of human Speedy/Ringo.|
|Purification||Antigen affinity chromatography|
|Storage buffer||tris citrate, pH 7-8|
|Contains||0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
PA1-16959 is specific for the ~36 kDa Speedy/Ringo protein (isoform 2).
After a longer exposure, a 31 kDa band can be detected (isoform 1).
There is also a non-specific band detected at ~58 kDa.Suggested positive control: mouse testis lysate, mouse testis protein.
Speedy/Ringo (Spy1) regulates the G1/S phase transition of the cell cycle by binding and activating CDC2, CDK2 and CDKN1B/KIP1. It mediates cell survival during the DNA damage process through activation of CDK2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: hSpy/Ringo A; MLZ-465; mSpy/Ringo A; Protein expressed in male leptotene and zygotene spermatocytes 465; Rapid inducer of G2/M progression in oocytes A; RINGO A; Ringo antibody; SPDY1 protein antibody; SPDYA; speedy A; Speedy A isoform antibody; speedy A1; speedy A2; speedy homolog 1; speedy homolog A; Speedy protein A; Speedy-1; speedy/ringo; spermatogenesis related protein Gs4; Spy antibody; Spy1; Spy1 antibody.
Gene Aliases: 4921517J08Rik; 4930548B21Rik; GS4; MLZ-465; RINGO3; RINGOA; SPDY1; SPDYA; SPY1
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