|Immunohistochemistry (IHC)||2.5 µg/ml|
|Western Blot (WB)||1-2 ug/ml|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Tested Species reactivity||Human, Mouse, Rat|
|Published species reactivity||Human Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A 20 amino acid peptide near the center of the human Spred1.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
A suggested positive control is mouse brain tissue lysate.
PA5-20617 can be used with blocking peptide PEP-0737.
The Ras-MAP kinase pathway is essential for the differentiation of neuronal cells and myocytes; it is inhibited by Spred1, a member of the Sprouty family of proteins. Spred1 acts by suppressing the phosphorylation and activation of Raf. The Spred proteins have also been implicated in the negative feedback regulation of FGF signaling in embryogenesis and angiogenesis. Further studies have shown that expression levels of Spred1 and Spred2 proteins are inversely correlated with the incidence of tumor invasion and metastasis in human hepatocellular carcinoma (HHC), suggesting that these proteins could be useful as prognostic factors and therapeutic targets in HCC. Defects in this gene are a cause of neurofibromatosis type 1-like syndrome (NFLS). This Spred1 antibody is predicted to have no cross-reactivity to Spred2 or Spred3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: protein phosphatase 1, regulatory subunit 147; Spred-1; sprouty protein with EVH-1 domain 1, related sequence; sprouty related, EVH1 domain containing 1; sprouty-related protein 1 with EVH-1 domain; sprouty-related, EVH1 domain containing 1; Sprouty-related, EVH1 domain-containing protein 1; suppressor of Ras/MAPK activation
Gene Aliases: 5730461F13Rik; AL024345; AW047647; hSpred1; NFLS; PPP1R147; spred-1; SPRED1
Molecular Function: receptor
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