|Flow Cytometry (Flow)||1:100|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Western Blot (WB)||1:25|
|Tested Species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from the internal region of human stathmin|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is tonsil or breast carcinoma.
Stathmin is a ubiquitous cyotsolic phosphoprotein proposed to function as an intracellular relay integrating regulatory signals of the cellular environment. The encoded protein is involved in the regulation of the microtubule filament system by destabilizing microtubules. It prevents assembly and promotes disassembly of microtubules. Alternattively spliced transcript variants encoding the same protein have been identified.
Protein Aliases: C1orf215; Lag; LAP18; leukemia associated phosphoprotein p18; Leukemia-associated gene protein; Leukemia-associated phosphoprotein p18; Metablastin; Oncoprotein 18; OP18; phosphoprotein 19; Phosphoprotein p19; pp17; PP19; PR22; Prosolin; Protein Pr22; SMN; Stathmin; stathmin 1/oncoprotein 18; testicular tissue protein Li 189; transmembrane protein C1orf215
Gene Aliases: 19k; C1orf215; Lag; LAP18; OP18; P18; P19; Pig; PP17; Pp18; PP19; PR22; SMN; STMN1