|Western Blot (WB)||1:500 - 1:1000|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues E(24) S N G P V K V W G S I K(36) of human superoxide dismutase (SOD1).|
|Storage buffer||whole serum|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-18274 detects Superoxide Dismutase 1 from human samples.
PA1-18274 has been successfully used in Western blot procedures.
The PA1-18274 immunogen is a synthetic peptide corresponding to residues E(24) S N G P V K V W G S I K(36) of human superoxide dismutase (SOD1), conjugated to diphtheria toxoid.
After reconstitution keep aliquots at -20°C for a higher stability, and at 4°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid freeze/thaw cycles.
Reconstitute with 100 ul of distilled water.
Superoxide dismutase (SOD) is an antioxidant enzyme involved in the defense system against reactive oxygen species (ROS). SOD catalyzes the dismutation reaction of superoxide radical anion (O2) to hydrogen peroxide, which is then catalyzed to innocuous O2 and H2O by glutathione peroxidase and catalase. Several classes of SOD have been identified. These include intracellular copper, zinc SOD (Cu, Zn-SOD/SOD-1), mitochondrial manganese SOD (Mn-SOD/SOD-2) and extracellular Cu, Zn-SOD (EC-SOD/SOD-3) (1). SOD1 is found in all eukaryotic species as a homodimeric 32 kDa enzyme containing one each of Cu and Zn ion per subunit (2). The manganese containing 80 kDa tetrameric enzyme SOD2, is located in the mitochondrial matrix in close proximity to a primary endogenous source of superoxide, the mitochondrial respiratory chain (3). SOD3 is a heparin-binding multimer of disulfide-linked dimers, primarily expressed in human lungs, vessel walls and airways (4). SOD4 is a copper chaperone for superoxide dismutase (CCS), which specifically delivers Cu to copper/zinc superoxide dismutase. CCS may activate copper/zinc superoxide dismutase through direct insertion of the Cu cofactor
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ALS1; Cu/Zn superoxide dismutase; epididymis secretory protein Li 44; Homodimer; hSod1; Indophenoloxidase A; IPOA; SOD, soluble; SOD1; Superoxide dismutase 1; Superoxide dismutase [Cu-Zn]; superoxide dismutase, cystolic
Gene Aliases: ALS; ALS1; HEL-S-44; homodimer; hSod1; IPOA; SOD; SOD1
UniProt ID: (Human) P00441
Entrez Gene ID: (Human) 6647
Molecular Function: oxidoreductase
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