|Immunocytochemistry (ICC)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||5 µg/ml|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1-2 µg/ml, 60 min|
|Western Blot (WB)||See 1 publications below|
|Tested Species reactivity||Human, Mouse, Rat|
|Published species reactivity||Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant fragment (aa 5-360) of human Syk.|
|Storage buffer||PBS, pH 7.4|
|Contains||15mM sodium azide|
|Storage conditions||4° C, do not freeze|
Syk is a cytoplasmic protein tyrosine kinase that translocates to the plasma membrane upon B cell antigen receptor (BCR) or the high-affinity IgE receptor (FcepsilonRI) triggering, and phosphorylates downstream adaptor proteins, thereby providing docking sites for initiation of subsequent signaling pathways, such as calcium mobilization, cytoskeleton remodeling, or transcription of specific genes. Syk binds to the receptor assemblies through interactions of its pair of SH2 domains with ITAM motives of the receptor, which have been phosphorylated by Src-family kinases. These kinases also help to activate Syk by phosphorylation of its activation loop.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: p72-Syk; p72Syk; Protein tyrosine kinase p72Syk; Spleen tyrosine kinase; Tyrosine-protein kinase SYK
Gene Aliases: p72-Syk; p72syk; ptk72; SYK; Sykb
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