|Immunofluorescence (IF)||Assay dependent|
|Western Blot (WB)||1:5000|
|Western Blot (WB)||See 2 publications below|
|Immunocytochemistry (ICC)||See 1 publications below|
|Tested Species reactivity||Bovine, Mouse, Sheep, Rat|
|Published species reactivity||Rat Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues G(253) P G G Y G P Q D S Y G P Q G G Y Q P D(272) of rat Synaptophysin.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-1043 detects Synaptophysin in rat, mouse, bovine, and sheep samples.
PA1-1043 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects a ~33.8 kDa protein representing Synaptophysin in rat, mouse and bovine brain tissue as well as sheep retinas.
The PA1-1043 immunogen is a synthetic peptide corresponding to residues G(253) P G G Y G P Q D S Y G P Q G G Y Q P D(272) of rat Synaptophysin. This sequence is conserved in many species including human, mouse and bovine. The PA1-1043 immunizing peptide (Cat. # PEP-279) is available for use in neutralization and control experiments.
Synaptophysin is a calcium-binding and integral membrane glycoprotein present in presynaptic vesicles in almost all neurons. Synaptophysin has four transmembrane domains and it forms a complex with dynamin at high calcium concentrations suggesting an involvement in synaptic vesicle endocytosis.
Synaptophysin is currently the most widely used marker for nerve terminals and for differentiating neuroendocrine tumors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: BM89 antigen; Major synaptic vesicle protein p38; Synaptophysin; Syp I
Gene Aliases: A230093K24Rik; AI848995; BOS_25912; p38; Syn; SYP; Syp1
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