|Immunohistochemistry (Paraffin) (IHC (P))||1:200|
|Western Blot (WB)||1:100|
|Immunohistochemistry (Frozen) (IHC (F))||See 1 publications below|
|Immunohistochemistry (Paraffin) (IHC (P))||See 1 publications below|
|Miscellaneous PubMed (MISC)||See 3 publications below|
|Immunohistochemistry (IHC)||See 18 publications below|
|Immunocytochemistry (ICC)||See 1 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Human , Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to C-terminus of human synaptophysin.|
|Storage buffer||tissue culture supernatant diluted in TBS, pH 7.5, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is pancreas or neuroendocrine tumor.
This antibody recognizes a protein of 38kDa, identified as synaptophysin. It labels normal neuroendocrine cells of human adrenal medulla, carotid body, skin, pituitary gland, thyroid, lung, pancreas, gastrointestinal mucosa, Paneth and quote;s cells in the gastrointestinal tract and of gastric parietal cells. Neurons in the brain, spinal cord, and retina are also labeled. Anti-synaptophysin antibody reacts with neuroendocrine neoplasms of neural as well as epithelial types e.g. neuroblastomas, ganglioneuroblastomas, ganglioneuromas, pheo-chromocytomas, chromaffin, and non-chromaffin paragangliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Major synaptic vesicle protein p38; MRXSYP; Synaptophysin
Gene Aliases: MRX96; MRXSYP; SYP
UniProt ID: (Human) P08247
Entrez Gene ID: (Human) 6855
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