|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
|Tested Species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 15 and 279 of Human TAFII68|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA5-27845 targets TAFII68 in IF, IHC (P), and WB applications and shows reactivity with Human and mouse samples.
The PA5-27845 immunogen is recombinant fragment corresponding to a region within amino acids 15 and 279 of Human TAFII68.
Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes a subunit of TFIID present in a subset of TFIID complexes. Translocations involving chromosome 17 and chromosome 9, where the gene for the nuclear receptor CSMF is located, result in a gene fusion product that is an RNA binding protein associated with a subset of extraskeletal myxoid chondrosarcomas. Two transcripts encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 68 kDa TATA-binding protein-associated factor; 68kD (RNA-binding protein 56); hTAFII68; N; Npl3; RBP56; RBP56/CSMF fusion; RNA polymerase II; RNA-binding protein 56; TAF(II)68; TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 68 kDa; TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 68kDa; TAF2N; TATA box binding protein (TBP)-associated factor; TATA box binding protein (TBP)-associated factor, RNA polymerase II, N, 68kD (RNA binding protein 56); TATA box binding protein (TBP)-associated factor, RNA polymerase II, N, 68kD (RNA-binding protein 56); TATA box binding protein associated factor 15; TATA box-binding protein-associated factor 2N (RNA-binding protein 56); TATA-bin; TATA-binding protein-associated factor 2N; TBP-associated factor 15
Gene Aliases: 2610111C21Rik; 68kDa; Npl3; RBP56; TAF15; TAF2N; TAFII68
UniProt ID: (Human) Q92804
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