|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:200|
|Western Blot (WB)||1:500-1:1000|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to amino acids 580-630 of human TCF-4|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.2|
|Contains||0.05% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
This antibody detects endogenous protein at a molecular weight of 60 and 72 kDa.
Purity is >95% by SDS-PAGE.
T cell factors (TCFs) comprise a family of DNA-binding transcriptional activators that are essential for lymphoid cell development. These transcription factors are activated by the Wnt-1 and Wingless pathways and are characterized by the presence of a conserved protein motif, the high mobility group(HMG) 1 box, which mediates DNA binding. TCF-4 mainly localizes in the cytoplasm and is transported into the nucleus directly bound to beta-catenin in a cooperative manner. This TCF-4/ beta-catenin complex induces expression of Wnt target genes, including multiple cancer-associated genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: BHLHB19; BHLHB20; Class A helix-loop-helix transcription factor ME1; Class A helix-loop-helix transcription factor ME2; Class B basic helix-loop-helix protein 19; Class B basic helix-loop-helix protein 20; DNA-binding protein HTF4; E-box-binding protein; HEB; helix-loop-helix transcription factor 4; HTF4; Immunoglobulin transcription factor 2; immunoglobulin transcription factor-2; ITF-2; ITF2; MITF-2; R8f DNA-binding protein; RITF-2; Salivary-specific cAMP response element-binding protein alpha; SCBP alpha; SCBP-alpha; SEF-2; SEF2; SL3-3 enhancer factor 2; TCF-12; TCF-4; TCF12; TCF4; Transcription factor 12; Transcription factor 4; Transcription factor 4 (Immunoglobulin transcription factor 2) (ITF-2) (MITF-2) (SL3-3 enhancer factor 2) (SEF-2) (Class A helix-loop-helix transcription factor ME2); transcription factor 4, isoform C; transcription factor 4, isoform D; transcription factor 4, isoform E; transcription factor 4, isoform L; transcription factor 4, isoform M; transcription factor 4, isoform R; Transcription factor HTF-4
Gene Aliases: 5730422P05Rik; A130037E08Rik; ALF1; ASP-I2; BHLHB19; BHLHB20; CRS3; E2-2; E2.2; FECD3; HEB; HEBAlt; HsT17266; HTF-4; HTF4; ITF-2; ITF-2b; ITF2; ME1; ME2; Meb; MITF-2A; MITF-2B; PTHS; REB; SCBPA; SEF-2; SEF2; SEF2-1; SEF2-1A; SEF2-1B; SEF2-1D; TCF-12; TCF-4; TCF12; TCF4; TFE
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