|Flow Cytometry (FACS)||1-2 ug/ml|
|Functional Assay (FN)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay-Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay-Dependent|
|Immunoprecipitation (IP)||1-2 ug/ml|
|Tested Species reactivity||Mouse|
|Host / Isotype||Armenian hamster / IgG|
|Immunogen||Affinity purified TCR from DO-11.10 T cell hybridoma|
|Storage buffer||PBS, pH 7.4|
|Contains||15mM sodium azide|
|Storage conditions||4° C, do not freeze|
This antibody reacts specifically with the beta subunit of TCR alpha/beta. It does not cross-react with TCR gamma/delta type and has been used as TCR alpha/beta phenotypic marker.
Purity is > 95% by SDS-PAGE.
T cell receptors recognize foreign antigens which have been processed as small peptides and bound to major histocompatibility complex (MHC) molecules at the surface of antigen presenting cells (APC). Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. In a single cell, the T cell receptor loci are rearranged and expressed in the order delta, gamma, beta, and alpha. If both delta and gamma rearrangements produce functional chains, the cell expresses delta and gamma. If not, the cell proceeds to rearrange the beta and alpha loci. This region represents the germline organization of the T cell receptor beta locus. The beta locus includes V (variable), J (joining), diversity (D), and C (constant) segments. During T cell development, the beta chain is synthesized by a recombination event at the DNA level joining a D segment with a J segment; a V segment is then joined to the D-J gene. The C segment is later joined by splicing at the RNA level. Recombination of many different V segments with several J segments provides a wide range of antigen recognition. Additional diversity is attained by junctional diversity, resulting from the random additional of nucleotides by terminal deoxynucleotidyltransferase. Several V segments of the beta locus are known to be incapable of encoding a protein and are considered pseudogenes. The beta locus also includes several trypsinogen genes. [provided by RefSeq, Jul 2008]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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