|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:200|
|Western Blot (WB)||1:500-1:1000|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to the N-terminal of human TFIIH p44|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.2|
|Contains||0.05% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
This antibody detects endogenous protein at a molecular weight of 44 kDa.
Purity is >95% by SDS-PAGE.
Initiation of transcription from protein-coding genes in eukaryotes is a complex process that requires RNA polymerase II, as well as families of basal transcription factors. Binding of the factor TFIID (TBP) to the TATA box is believed to be the first step in the formation of a multiprotein complex containing several additional factors, including TFIIA, TFIIB, TFIIE, TFIIF and TFII. TFIIH (or BTF2) is a multisubunit transcription/DNA repair factor that possesses several enzymatic activities. The core of TFIIH is composed of five subunits, designated p89 (XPB or ERCC3), p62, p52, p44 and p34. Additional subunits of the TFIIH complex are p80 (XPD or ERCC2) and the ternary kinase complex composed of Cdk7, cyclin H and MAT1. Both p89 and p80 have ATP-dependent helicase activity. The p62, p52 and p44 subunits have been shown to be involved in nucleotide excision repair.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: basal transcription factor 2, p44 subunit; Basic transcription factor 2 44 kDa subunit; BTF2 p44; BTF2-p44; BTF2P44; general transcription factor II H, polypeptide 2 (44 kDa subunit); General transcription factor IIH polypeptide 2; General transcription factor IIH subunit 2; general transcription factor IIH, polypeptide 2 (44 kDa subunit); general transcription factor IIH, polypeptide 2, 44kD subunit; general transcription factor IIH, polypeptide 2, 44kDa; GTF2H2; TFIIH basal transcription factor complex p44 subunit
Gene Aliases: 44kDa; BTF2; BTF2 p44; BTF2-p44; BTF2P44; GTF2H2; p44; T-BTF2P44; TFIIH
Molecular Function: transcription factor
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.