|Immunohistochemistry (Paraffin) (IHC (P))||10 µg/ml|
|Western Blot (WB)||1-4 µg/ml|
|Tested Species reactivity||Human, Mouse, Non-human primate, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues 140-190 of human TIGAR.|
|Storage buffer||PBS with 0.2% gelatin|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
TIGAR a 270 AA, p53-inducible protein is known an isomerase belonging to phosphoglycerate mutase family. It is a part of the p53 tumor suppressor pathway that regulates glycolysis and protects against oxidative stress. Report shows that it has sequence similarity to the bisphosphate domain of the glycolytic enzyme that degrades fructose-2, 6-bisphosphate and blocks glycolysis thereby directing the pathway into the pentose phosphate shunt. Over expression of TIGAR in mouse and human cells resulted in inhibition of glycolysis, as well as a decrease in intracellular reactive oxygen species (ROS) and provides some protection from DNA damage-induced apoptosis in a cell type-specific manner.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Fructose-2,6-bisphosphatase TIGAR; fructose-2,6-bisphosphate 2-phosphatase; probable fructose-2,6-bisphosphatase TIGAR; TP53-induced glycolysis and apoptosis regulator; TP53-induced glycolysis regulatory phosphatase; transactivated by NS3TP2 protein
Gene Aliases: 9630033F20Rik; AA793651; AI595337; C12orf5; C79710; C85509; FR2BP; TIGAR
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