|Immunocytochemistry (ICC)||1-2 µg/ml|
|Immunofluorescence (IF)||1-2 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:100|
|Western Blot (WB)||1:250-1:1000|
|Immunohistochemistry (IHC)||See 1 publications below|
|Tested Species reactivity||Human, Mouse, Rhesus monkey|
|Published species reactivity||Mouse|
|Host / Isotype||Mouse / IgG1|
|Clone||F31 P2 A5|
|Immunogen||Synthetic peptide corresponding to residues F(72) Q A L G D A A D I R(82) of mouse TIMP-1.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA1-773 contains 100 ug of in vitro produced, protein A purified antibody (1 mg/ml) in PBS containing 1 mg/ml BSA and 0.05% sodium azide.
MA1-773 detects TIMP-1 from human samples.
MA1-773 has been successfully used in Western blot, immunofluorescence, and immunohistochemistry procedures. By Western blot, MA1-773 detects a ~30 kDa band representing TIMP-1 from K562 cell lysate. It is recommended to block with 3% BSA.
The MA1-773 immunogen is a synthetic peptide corresponding to residues F(72) Q A L G D A A D I R(82) of mouse TIMP-1.This sequence is 100% conserved in some non-human primate TIMP-1.
Tissue inhibitor of metalloproteinases-1 (TIMP-1) and Tissue inhibitor of metalloproteinases-2 (TIMP-2) are inhibitory enzymes of matrix metalloproteinase family. TIMP-1 forms a complex of 1:1 stoichiometry with activated interstitial collagenase, activated stromelysin, active form of 72 kD Type IV collagenase (MMP-2) and latent and active forms 92 kD Type IV collagenase (MMP-9). TIMPs inhibit the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Collagenase inhibitor; Collagenase inhibitor 16C8 fibroblast; EPA; erythroid potentiating activity; Erythroid-potentiating activity; Fibroblast collagenase inhibitor; Metalloproteinase inhibitor 1; TIMP-1; TIMP1; tissue inhibitor of matrix metalloproteinase-1; tissue inhibitor of metalloproteinase-1; Tissue inhibitor of metalloproteinases 1; TPA-induced protein; TPA-S1
Gene Aliases: CLGI; EPA; EPO; HCI; TIMP; TIMP-1; TIMP1; TPA-S1
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.