|Flow Cytometry (Flow)||2-5 µg/1x10^6 cells|
|Tested Species reactivity||Dog, Guinea pig, Human, Mouse, Non-human primate, Pig|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Ba/F3 cell line expressing human TLR4 cell surface antigen|
|Storage buffer||50mM sodium borate|
|Contains||0.05% sodium azide|
|Storage conditions||4° C, store in dark|
This antibody has been demonstrated to block activation of monocytes with LPS.
Endotoxin Level is <0.01 EU/ug.
Suggested positive control: human PBMCs. This antibody is not recommended for Western Blot applications.
The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). Among this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediators of LPS signaling (4,5). The TLR4 cDNA codes for a protein consisting of 799 amino acids with approximate molecular weight of 88 kDa (6).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ARMD10; CD284; homolog of Drosophila toll; hToll; TLR-4; TOLL; Toll-like Receptor 4; Toll-like receptor4 protein
Gene Aliases: ARMD10; CD284; TLR-4; TLR4; TOLL
UniProt ID: (Human) O00206
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