|Flow Cytometry (Flow)||2-5 µg for 10^6 cells|
|Immunohistochemistry (Frozen) (IHC (F))||Assay dependent|
|Western Blot (WB)||1-3 µg/ml|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide, conjugated to KLH, corresponding to residues L(706) S H N Q L T T V P E R L S N C S R S L K N L(728) of human TLR7.|
|Contains||0.05% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
PA1-28109 detects TLR7 from human samples. PA1-28109 is expected to cross-react with rat and mouse due to sequence homology (86% identity with immunogen for both species).
PA1-28109 has been successfully used in immunohistochemistry (frozen tissue), flow cytometry and Western blot applications.
The PA1-28109 immunogen is a synthetic peptide, conjugated to KLH, corresponding to residues L(706) S H N Q L T T V P E R L S N C S R S L K N L(728) of human TLR7.
The Toll like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL 1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules and could constitute an important and unrecognized component of innate immunity in humans. The TRL family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. TRLs characterized so far activate the MyD88/interleukin 1 receptor-associated kinase (IRAK) signaling pathway. Ten human homolgs of TLRs (TLR1 10) have ben described. Stimulation of the NFkB signaling pathway by TLR7 suggest that it plays a role in immune response.
Protein Aliases: Toll-like receptor 7; toll-like receptor 7-like
Gene Aliases: TLR7; TLR7-like; UNQ248/PRO285