|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:200|
|Western Blot (WB)||2 µg/ml|
|Tested Species reactivity||Bovine, Chicken, Human, Mouse, Non-human primate, Rabbit, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic internal peptide corresponding to residues 100-200 of the human TMP21 protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 30% glycerol|
|Contains||0.1% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
This sequence is 100% conserved withing monkey, mouse, hamster, rat, rabbit, and bovine TMP21.
Suggested positive control: human liver protein.
TMP21 is a ubiquitously expressed protein that is involved in vesicular targeting and protein transport. More recent experiments have shown that it is also a component in the presenilin complex and modulates the gamma-secretase but not the epsilon-secretase cleavage activity of the amyloid precursor protein. The presenilin complex is composed of the proteins APH1, nicastrin, and PEN2 in addition to presenilin-1. Together, these proteins cleave the amyloid precursor protein at what is known as the gamma- and epsilon-sites and can lead to the accumulation of the Abeta cleavage product that is associated with Alzheimer and quote;s disease.
Co-immunoprecipitation experiments using antibodies against these proteins also yielded TMP21 indicating that TMP21 may play a role in the regulation of this complex. Suppression of TMP21 expression by siRNA in transfected cells caused increased gamma-secretase activity but not epsilon-secretase activity, and increased Abeta production, demonstrating that TMP21 can modulate gamma-secretase activity.
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Protein Aliases: 21 kDa transmembrane trafficking prote; 21 kDa transmembrane trafficking protein; 21 kDa transmembrane-trafficking protein; Integral membrane protein p23; integral membrane protein Tmp21-I (p23); p23; p24 family protein delta-1; p24delta; p24delta1; S31I125; S31III125; testicular tissue protein Li 206; Tmp-21-I; Transmembrane emp24 domain-containing protein 10; transmembrane emp24-like trafficking protein 10; Transmembrane protein Tmp21; transmembrane trafficking protein; transmembrane trafficking protein 21
Gene Aliases: 1110014C03Rik; BOS_11050; p23; P24(DELTA); p24d1; p24delta1; RCJMB04_24a4; S31I125; S31III125; TMED10; Tmp-21-I; TMP21
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